Cellular physiology

Achievements

The core facility regularly develops and validates new tests and assays in association with the research teams and their collaborators (see table below).   

For example, we have been able to miniaturize many assays to fit small volumes of preclinical rodent samples.  

The core facility has also developed with an industrial partner new immunoassays for non-commercially available targets (e.g. glucagon). 

If you have a development idea that you think would be of interest to different users, please contact the core facility: physio.cell.cr.chum@ssss.gouv.qc.ca 

New developments in the cellular physiology core facility since 2014
Year of implementation Test or application  Technological approach used 
2021 Cholesterol assay in cell lines Enzyme assay and cholic acid extraction
2020 Morphometry of adipocytes   HE staining and Adiposoft software 
2019 Human Il-2 assay  TR-FRET 
2019 Determination of cellular triglyceride content   Enzyme assay
2019 Mouse CRP assay  ELISA 
2018 Creatinine measurement  Enzyme assay
2018 Miniaturization of urea assay Enzyme assay
2018 Morphometry of the islets of Langerhans  Immunohistochemistry and high definition scanner
2018 Miniaturization of fructose determination Enzyme assay 
2018 Miniaturization of triglyceride determination Enzyme assay
2018 Miniaturization of free fatty acid determination  Enzyme assay 
2018 Miniaturization of lactate determination Enzyme assay
2018 Miniaturization of plasma cholesterol measurement Enzyme assay
2018 Estradiol assay  ELISA 
2018 Testosterone assay AlphaLISA 
2017 Tissue triglyceride assay Enzyme assay after saponification
2017 Fructose assay Enzyme assay
2017 Somatostatin assay  AlphaLISA 
2017 Corticosterone determination AlphaLISA 
2014 Immunohistochemistry ratio of the / cells of the islets of Langerhans  Immunohistochemistry and high definition scanner
2014 Ki-67 immunohistochemistry of the islets of Langerhans   Immunohistochemistry and high definition scanner
2014 Chromogranin immunohistochemistry of the islets of Langerhans  Immunohistochemistry and high definition scanner

 

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